| 李桂芬1;朱水芳1;周群2;施宗伟1;张永江1;李明福1.侵染紫松果菊的黄瓜花叶病毒分离物鉴定[J].植物保护,2007,33(1):54-56. |
| 侵染紫松果菊的黄瓜花叶病毒分离物鉴定 |
| Identification of Cucumber mosaic virus isolated from Echinacea purpurea |
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| DOI： |
| 中文关键词: 紫松果菊 黄瓜花叶病毒 鉴定 |
| 英文关键词:Echinacea purpurea Moench Cucumber mosaic virus identification |
| 基金项目: |
| 李桂芬1;朱水芳1;周群2;施宗伟1;张永江1;李明福1 |
| 1.中国检验检疫科学研究院动植物检验检疫研究所 北京100024;2.山东省莱西市农业局植保站 266600 |
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| 中文摘要: |
| 从表现黄花叶症状的紫松果菊病株上获得分离物2-1-2,电镜下可见直径约30 nm的球状病毒粒体,其与黄瓜花叶病毒抗体呈强的阳性反应,ds-RNA的谱带类型与本实验室保存的标准黄瓜花叶病毒株系相同。通过生物学、病毒粒体观察、血清学以及病毒核酸双链试验结果,确定该病毒分离物为黄瓜花叶病毒。 |
| 英文摘要: |
| The virus particles of about 30nm in diameter were isolated from Echinacea purpurea Moench showing systemic sympotoms,including yellow mosaic on leaves.The virus-infected plants reacted positively with the Cucumber mosaic virus(CMV) antibody in triple antibody sandwich enzyme-linked immunosorbent assay(TAS-ELISA).The type of the double-stranded RNA pattern of the isolate was the same as the standard CMV.On the basis of the biology,particle morphology,serology and ds-RNA pattern of the virus,it was concluded that the virus isolated from E.purpurea in Huairou,Beijing is Cucumber mosaic virus. |
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