| 高宗军1,蒋家珍1,李学锋1,邱立红1,王成菊1,张文吉1,程玉臣2.吡唑解草酯对小麦细胞色素P450的诱导作用及其光谱特征[J].植物保护,2005,31(4):40-44. |
| 吡唑解草酯对小麦细胞色素P450的诱导作用及其光谱特征 |
| Studies on the induction of wheat cytochrome P450 by mefenpyr-diethyl and the spectrum characteristics |
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| DOI： |
| 中文关键词: 农药学 吡唑解草酯 细胞色素P450 细胞色素P420 诱导作用 |
| 英文关键词:pesticide science mefenpyr-diethyl cytochrome P450 cytochrome P420 induction |
| 基金项目: |
| 高宗军1 蒋家珍1 李学锋1 邱立红1 王成菊1 张文吉1 程玉臣2 |
| 1.中国农业大学应用化学系 北京100094;2.内蒙古农业科学院植物保护研究所 呼和浩特010000 |
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| 中文摘要: |
| 用吡唑解草酯浇灌小麦,试验结果表明,50μmol/L吡唑解草酯处理抗6号小麦,可使其细胞色素P450含量达到最大值108.18 pmol/mg蛋白质,为对照组的1.67倍;100μmol/L吡唑解草酯处理敏18号小麦,可使其细胞色素P450含量达到最大值80.97 pmol/mg蛋白质,为对照组的1.86倍。吡唑解草酯对两个小麦品种的细胞色素P450均有诱导作用,抗6号小麦更容易被诱导,这与两小麦品种的耐药性一致。室温(20±1)℃下扫描不同时间的细胞色素P450-CO结合光谱,结果表明,微粒体粗提液室温(20±1)℃保存200 min后,细胞色素P450完全转变为细胞色素P420。 |
| 英文摘要: |
| After treatment of R-6# wheat(a tolerant wheat) with 50μmol/ L mefenpyr-diethyl,its cytochrome P450 content was up to 108.18 pmol/mg protein,which is 1.67 times as the control's.100μmol/ L mefenpyr-diethyl raised the cytochrome P450 content of S-18# wheat(a sensitive wheat) by 86%,up to the maximum 80.97 pmol/mg protein,compared to that of the control.The results indicated that mefenpyrdiethyl could induce cytochrome P450 in two of the wheat cultivars,but R-6# is more susceptible to induction than S-18#,which is in agreement with the results obtained by herbicide treatment.After crude microsomal preparation stored at room temperature(20±1)℃ for 200 minutes,the cytochrome P450 was entirely transformed into the inactive form,cytochrome P420. |
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