| 刘太国;吴茂森;张文蔚;李世访;成卓敏.花粉管通道法介导的抗大麦黄矮病毒转基因小麦研究[J].植物保护,2005,31(4):32-35. |
| 花粉管通道法介导的抗大麦黄矮病毒转基因小麦研究 |
| Transgenic wheat resistant to Barley Yellow Dwarf Virus obtained via pollen tube pathway |
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| DOI： |
| 中文关键词: 基因工程 大麦黄矮病毒 花粉管通道法 复制酶基因 |
| 英文关键词:genetic engineering Barley Yellow Dwarf Virus pollen tube pathway replicase gene |
| 基金项目: |
| 刘太国;吴茂森;张文蔚;李世访;成卓敏 |
| 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 北京100094 |
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| 中文摘要: |
| 构建了大麦黄矮病毒GPV株系串联结构复制酶基因植物表达载体pPPI29,通过花粉管通道法转化了CA8686小麦,对T0代种子进行了分子检测,获得6株转基因小麦,转化率达到0.45%;对获得的T0代转基因小麦进行了田间抗病性鉴定,结果表明4株转基因小麦对GPV株系表现为高抗,可以推迟发病23 d。 |
| 英文摘要: |
| The cDNA of the direct repeat replicase genes of BYDV-GPV was obtained by RT-PCR and was inserted into the plasmid to construct an expression plasmid named pPPI29.CA8686 was then transformed with pPPI29 via pollen tube pathway.Six positive transgenic wheat plants were confirmed by Dot-plot,PCR and PCR-Southern assay among 1343 plants and the transformation frequency was 0.45%.The resistance evaluation of T0 generation of the transgenic wheat plants carrying GPV replicase gene were carried out.Compared with non-transgenic plants,four transgenic lines of CA8686 showed higher level of resistance after virus infection.They could delay symptom development for about 23 days. |
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