| 宋震;徐小峰;杨方云;周常勇.柑橘衰退病毒柚分离株的p25/Hinf1 RFLP分析[J].植物保护,2005,31(4):28-31. |
| 柑橘衰退病毒柚分离株的p25/Hinf1 RFLP分析 |
| A p25/Hinf1 RFLP analysis of the Citrus tristeza virus isolates infecting pummelo |
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| DOI： |
| 中文关键词: 植物病毒学 柑橘衰退病毒 分离株 限制性片段长度多态性分析 |
| 英文关键词:phytovirology Citrus Tristeza Virus isolate RFLP assay |
| 基金项目: |
| 宋震;徐小峰;杨方云;周常勇 |
| 中国农业科学研究院柑橘研究所 重庆400712 |
| 摘要点击次数: 703 |
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| 中文摘要: |
| 对123个柑橘衰退病毒柚分离株进行p25/Hinf1 RFLP分析明确:样品中,单一p25/Hinf1 RFLP组群样品占样品总量的82.9%,说明我国田间受侵染柚类中柑橘衰退病毒株系相对较为单一;RFLP组群6的样品占样品总量的79.7%,说明目前柚类生产上的优势流行株属于p25/Hinf1 RFLP组群6,初步认定为强毒株系;柑橘衰退病毒株柚分离株S102、S104、S106、S108属于p25/Hinf1 RFLP组群5,初步认定是弱毒株系。 |
| 英文摘要: |
| Citrus Tristeza Virus(CTV) is one of the most economically important pathogen of citrus in many citrus producing areas of the world.Pummelo dwarf caused by CTV has become a serious problem in China recently.In order to discover their pathogenic characteristics,123 CTV isolates infecting pummelo were studied by p25/Hinf1 RFLP assay.Single p25/Hinf1 RFLP groups were detected in 82.9% of the 123 samples,and the dominant group was p25/Hinf1 RFLP group 6,which accounted for 79.7% of the total.There were 4 isolates belonging to p25/Hinf1 RFLP group 5,which could be mild CTV strains and should be further studied. |
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