| 王清1,李长福1,吴振廷1,郑厚今2,王学林1,林华峰1.种子生测法鉴定转Bt基因棉抗虫性[J].植物保护,2004,30(4):24-28. |
| 种子生测法鉴定转Bt基因棉抗虫性 |
| Insect resistance to Bt-transgenic cotton by seed bioassay |
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| DOI： |
| 中文关键词: 抗病虫育种 种子生测法 转Bt基因棉 抗性鉴定 |
| 英文关键词:resistance breeding to pests seed bioassay Bt-transgenic cotton identification |
| 基金项目: |
| 王清1 李长福1 吴振廷1 郑厚今2 王学林1 林华峰1 |
| 1.安徽农业大学植物保护系 合肥 230036;2.安徽省种子总公司 合肥 230036 |
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| 中文摘要: |
| 通过室内应用单粒种子生测法鉴定转Bt基因棉抗虫性,明确了单粒棉种室内生测鉴定转Bt基因棉抗虫性的最佳生测条件。将转Bt基因棉种子25℃萌动72 h后匀浆,与0.45 g饲料混匀,以2日龄棉铃虫幼虫为试虫,测定其死亡率。建立了相应的抗性标准:棉铃虫幼虫校正死亡率小于25％为中抗,处于25％-50％为抗,50％-65％为高抗,大于65％为特高抗。准确性验证表明,种子生测法同叶片生测法的结果相符,具有较好的准确性。 |
| 英文摘要: |
| The bioassay with neonate larvae of bollworm was carried out to test their resistance to Bt-transgenic cotton using its seed. The results showed that the bioassay with 2nd instar larvae was appropriate by soaking a seed for 72 hours at 25℃, and mixing it with 0.45 g artificial foods. The evaluation criterion was established as follows: 25% or smaller larva mortality was medium resistance, 25%-50% resistance, 50%-65% high resistance, and 65% or above particularly high resistance. It was verified that the bioassay results by using cotton seeds of different insect resistance level agreed with those by using the leaves of Bt-transgenic cotton and this method was stable and reliable. |
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