| 黄星,周明国,张永慧,傅峰.稻瘟病菌对三环唑抗药性测定方法的建立[J].植物保护,2004,30(1):55-58. |
| 稻瘟病菌对三环唑抗药性测定方法的建立 |
| Approach to measuring resistance of Pyricularia grisea to tricyclazole |
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| DOI： |
| 中文关键词: 植物病理学 稻瘟病菌 三环唑 抗药性测定 |
| 英文关键词:phytopathology Pyricularia grisea tricyclazole measurement of fungcide resistance |
| 基金项目: |
| 黄星 周明国 张永慧 傅峰 |
| 南京农业大学农业部病虫监测与治理重点开放实验室 南京210095 |
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| 中文摘要: |
| 研究了稻瘟病菌对三环唑抗药性的测定方法,确定了保绿培养基的成分,建立了抗药性测定的标准化模型。4叶I心期将稻苗在三环唑系列浓度药液中浸根处理36 h,剪取稻苗叶段插于100 μg/mL苯并咪唑+0.5％水琼脂保绿培养基上,用孢子含量为105个/mL的稻瘟病菌孢子悬浮液喷雾接种,保湿培养8 d后调查结果,计算EC50值。该测定方法与小苗测定相比较结果更加稳定且灵敏度高,重复性好。 |
| 英文摘要: |
| The method of monitoring resistance of Pyricularia grisea to tricyclazole was studied and the model was set up. After root-soaking for 36 hours, the leave fragments of rice seedlings were cut and placed onto 0.5% agar with 100 μg/ mL benzimidazole. After artificially inoculated with spore suspension (105 spores/mL) , the leave fragments were put back to the moist climatic chamber for observation of disease symptoms for 8 days. Comparative studies showed that the approach described in the present paper was more stable and sensitive than the seedling treatment. |
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