| 张文蔚,成卓敏.NPTⅡ基因和GUS基因在小麦遗传转化中的应用[J].植物保护,2004,30(1):48-50. |
| NPTⅡ基因和GUS基因在小麦遗传转化中的应用 |
| Application of NPT Ⅱ gene and GUS gene to wheat transformation |
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| DOI： |
| 中文关键词: 基因工程 小麦 NPTⅡ GUS基因 农杆菌 |
| 英文关键词:genetic enginerine wheat: NPT Ⅱ gene GUS gene Agrobacterium tumefaciens |
| 基金项目: |
| 张文蔚 成卓敏 |
| 中国农业科学院植物保护研究所国家病虫害生物学重点实验室 北京100094 |
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| 中文摘要: |
| 对小麦遗传转化上常用的选择标记基因NPTⅡ的选择剂及GUS基因在幼胚愈伤组织中的转移进行了研究。结果表明用G418作为选择剂,浓度为20-30mg／L较为合适。幼胚愈伤组织与农杆菌共培养3d后,即可检测到GUS基因的瞬时表达。农杆菌LBA4404由500 mg／L羧卞青霉素抑制;EHA105、AGLI由500 mg／L头孢霉素抑制。而不能用卡那霉素。 |
| 英文摘要: |
| The selectable marker gene ( NPT Ⅱ ) that is usually used for wheat transformation was tested for the optimal selector. The results indicated that 20 - 30 mg/L G418 was suitable for selection of NPT Ⅱ gene. Transient expression of GUS gene was detected in the tissues after 3 days of co-culture with Agrobacterium tumefaciens. Agrobacterium strain LBA4404 was inhibited by 500 mg/L Carbenicillin; EHA105 and AGLI were inhibited by 500 mg/L Cefotaxime. |
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