| 付月君;梁爱华.三种强启动子和egt基因缺陷在重组核型多角体病毒杀虫剂研发中的意义[J].植物保护,2003,29(1):11-13. |
| 三种强启动子和egt基因缺陷在重组核型多角体病毒杀虫剂研发中的意义 |
| The sense of three strong promoters and egt-minus in research and development of recombinant NPV pesticides |
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| DOI： |
| 中文关键词: 基因工程 重组 生物杀虫剂 核型多角体病毒 egt基因 |
| 英文关键词:gene engineering recombination living insecticide nuclear polytedrosis virus ege gene |
| 基金项目: |
| 付月君;梁爱华 |
| 山西大学生物技术研究所 太原030006 |
| 摘要点击次数: 760 |
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| 中文摘要: |
| 核型多角体病毒 (nuclearpolyhedrosisvirus ,NPV)是鳞翅目昆虫的重要病原微生物。采用构建重组高效表达外源毒蛋白的 p10、ph、ocu基因的强启动子 ,以提高野生型NPV的毒力 ;探讨删除杆状病毒中的蜕皮甾体尿苷二磷酸葡糖转移酶 (ecdysteroidUDP-glucosyltraansferase ,EGT)基因对改良病毒杀虫剂的杀虫效果 ,为进一步完善重组NPV杀虫剂的研发和安全生产提供了理论基础 |
| 英文摘要: |
| Nuclear polyedrosis virus (NPV) is pathogenic to a number of lepidopteran insects. To improve the toxic effects of the wild-type NPV, this article introduces some powerful promoters-p10、ph、ocu genes' promoters, which are very important in recombinant toxin-expressing virus. The deletion of egt gene actually improves the pesticidal properties of the NPV. Insects infected by an egt-type virus display a character of reducing feeding and earlier death than these infected by wild type virus. All of these reports can be a theortical base for improving the research and use of recombinant NPV. |
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