宁红1,2;高荣1;王丽焕1;蒋辉2;刘如东3;刘世贵1.新月弯孢菌及其近似菌菌落形态和可溶性蛋白电泳图谱比较分析[J].植物保护,2002,28(5):9-12. |
新月弯孢菌及其近似菌菌落形态和可溶性蛋白电泳图谱比较分析 |
Comparative analysis of colony morphometries and protein electrophoresis patterns of Curvularia lunata (Walk) and close isolates in maize |
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DOI: |
中文关键词: 新月弯孢菌 菌落形态 蛋白质图谱 |
英文关键词:Curvularia lunata(Walk) colony morphometrics protein electrophoresis pattern |
基金项目: |
宁红1 2;高荣1;王丽焕1;蒋辉2;刘如东3;刘世贵1 |
1.四川大学生命科学学院 成都610064;2.四川省农业厅植物检疫站 成都610041;3.四川绵阳市植保植检站 绵阳621000 |
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中文摘要: |
通过对分离自玉米及其他寄主的 12个新月弯孢菌菌株和 5个近似菌株菌落形态及可溶性蛋白电泳谱比较分析 ,表明菌落形态在不同种之间存在明显的差异 ,同种之间也存在一定差异 ,但不稳定和存在变异。 17个菌株可溶性蛋白SDS聚丙烯酰胺凝胶电泳显示 ,Curvularia属在Rf值为 0.177处有一条该属的特征蛋白带 ,新月弯孢菌在Rf值为0.225处有一条该种的特征蛋白带 ,但新月弯孢菌蛋白带存在多样性 ,聚类分析将未能确定的 13号菌株定为不等弯孢菌 |
英文摘要: |
Comparative analysis of myceliae colony morphometrics and SDS polyacrylamide gel electrophoresis patterns of soluble proteins of 12 isolates of Curvularia lunata and 5 close isolates were conducted. Differences of colony morphometrics of 17 isolates existed between different species and the same species, but they were not steady and consistent. Significant differences of SDS-PAGE patterns existed between different species and the same species. There was a specific protein band for Curvularia at relative mobility value 0.177 (Rf 0.177) and a specific protein band for C. lunata at Rf 0 225 However protein bands were diversified in C. lunata. Cluster and analysis classified unidentified No 13 strain into C inaegualis. |
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