杨建卿,江彤,陈学平.烟草疫霉菌的培养及大量产生游动孢子囊和游动孢子方法的研究[J].植物保护,2001,27(4):12-14. |
烟草疫霉菌的培养及大量产生游动孢子囊和游动孢子方法的研究 |
Studies on the culture of Phytophthora nicotianae and the methods for producing zoosporangium and zoospore rapidly and enormously |
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DOI: |
中文关键词: 烟草疫霉菌 人工培养 游动孢子囊 游动孢子 |
英文关键词:Phytophthora nicotianae Breda artificial culture zoosporangium zoospore |
基金项目: |
杨建卿 江彤 陈学平 |
中国科学技术大学经济技术学院,合肥230052 |
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中文摘要: |
烟草疫霉菌(Phytophthora nicothianae)在燕麦培养基上生长良好 ,菌丝在滴加了土壤浸出液的皮氏培养液中 ,可在 3d内产生大量的游动孢子囊 ,游动孢子囊经低温处理后再培养 2 4h可产生大量游动孢子 |
英文摘要: |
Phytophthora nicotianae could grow very well on oatmeal glucose agar medium.The hygha was cultured in 30 ml Petri solution which put 2-3 drops of filtered soil suspension . In this kind of blended solution, the hyphea of P. nicotianae could preduce a great quantity of zoosporangium by 3 days. Zoosporangium could produce a great quantity of zoospore after treated under low temperature and culturing 24 hours once more. |
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