| 檀建新,陈忠义,张杰,黄大昉.产几丁质酶菌的分离鉴定及其抑菌作用的初步研究[J].植物保护,2001,27(2):1-3. |
| 产几丁质酶菌的分离鉴定及其抑菌作用的初步研究 |
| Isolation and identification of chitinolytic strains and preliminary study on the antifungal activity of its crude chitinases |
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| DOI： |
| 中文关键词: 环状芽孢杆菌 几丁质酶 分离 鉴定 抑菌作用 |
| 英文关键词:Bacillus circulans chitinases isolation identification antifungal activity |
| 基金项目: |
| 檀建新 陈忠义 张杰 黄大昉 |
| 中国农业科学院植物保护研究所,北京100094 |
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| 中文摘要: |
| 从北京地区的土样、砂样和水样中分离并纯化了产生几丁质酶的菌株 ,其中CT14含几丁质酶活性最高 ,经对其细胞形态、菌落特征的观察和生化特性检测 ,证明该菌株是环状芽孢杆菌 (Bacillus circulans)。其几丁质酶粗提液能抑制多种植物病原真菌的生长 ,表明它对植物病原真菌的拮抗作用具有广谱性。 |
| 英文摘要: |
| Several chitinolytic isolates were isolated from soil, sand, and water samples of Beijing. Among them the isolate CT14 was identified as Bacillus circulans that produced chitinase of the highest activity. The physiological characteristics of CT14, the morphological characteristics of CT14 cells and clones were studied. Its crude chitinases showed the inhibition against a wide range of plant pathogenic fungi. |
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