| 周妍茹, 孟虹百, 代思遥, 何文馨, 周崇杰, 常小丽*.木霉菌复配生物种衣剂的研制及对大豆根腐病的防效[J].植物保护,2026,(1):273-281. |
| 木霉菌复配生物种衣剂的研制及对大豆根腐病的防效 |
| Development of compound Trichoderma based biocontrol seed coating agents and their control efficacy against soybean root rot |
| 投稿时间：2024-12-18 修订日期：2025-01-29 |
| DOI：10.16688/j.zwbh.2024662 |
| 中文关键词: 木霉 分生孢子 生物种衣剂 大豆根腐病 防效 |
| 英文关键词:Trichoderma conidia biological seed coating agents soybean root rot control efficacy |
| 基金项目:国家重点研发计划(2023YFD1401000); 广西壮族自治区重点研发计划(AB23026107); 四川省重点研发计划(23ZDYF3037) |
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| 中文摘要: |
| 本文以前期从大豆根际筛选获得的根腐病高效抑病菌株非洲哈茨木霉Trichoderma afroharzianum RA1-31和哈茨木霉T. harzianum RA2-8为材料, 制备木霉菌分生孢子, 通过筛选菌株配比、成膜剂种类和包衣药种比, 获得复合型木霉种衣剂, 在室内和大田条件下测定其对大豆根腐病的防治效果。结果表明, 以木霉菌株RA1-31和RA2-8分生孢子粉作为原料, 按照分生孢子和高岭土质量比1∶3添加高岭土, 40℃烘干, 制得混合木霉菌分生孢子粉, 孢子萌发率可达90%以上; 以2个菌株质量比1∶1混合的分生孢子为活性成分, 2.0%酸溶性壳聚糖溶液为成膜剂, 制成质量浓度为50%的孢子粉悬浮液, 并按药种比1∶100对大豆种子包衣后播种, 在室内和大田盆栽条件下, 该复合种衣剂对大豆根腐病的防治效果分别达到63.90%和60.89%。本研究开发的木霉复配生物种衣剂对根腐病防治效果显著, 且安全、低毒, 具有进一步开发和应用的潜力。 |
| 英文摘要: |
| This study aimed to develop a compound Trichoderma-based seed coating agent for the control of soybean root rot. Two highly antagonistic strains, Trichoderma afroharzianum RA1-31 and T.harzianum RA2-8, previously isolated from the soybean rhizosphere, were used as the active ingredients. Conidial powders of both strains were prepared, and the optimal strain ratio, film-forming agents, and coating agent-to-seed ratio were determined to formulate the compound seed coating agent. The mixture of conidia was blended with kaolin at a mass ratio of 1∶3 and dried at 40℃, yielding a mixed conidial powder with a germination rate above 90%. The final formulation consisted of a 1∶1 mass ratio of conidia from the two Trichoderma strains and 2.0% acid-soluble chitosan solution as the film-forming agent, and a spore powder suspension with a mass concentration of 50%. The soybean seeds were cated at an agent-to-seed ratio of 1∶100 prior to sowing. Under laboratory and field pot conditions, the control efficacies of the compound seed coating agent against soybean root rot were 63.90% and 60.89%, respectively. The developed Trichoderma compound biocontrol seed coating agent exhibited significant control efficacy against soybean root rot, with the advantages of safety, low toxicity, and potential for large-scale development and application. |
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