| 茹川鉴1, 许光伟3, 王 琳3, 严善春1*, 刘 伟2*, 王桂荣2.橘小实蝇蛋白饵剂的活性引诱化合物鉴定及潜在应用[J].植物保护,2026,(1):204-213. |
| 橘小实蝇蛋白饵剂的活性引诱化合物鉴定及潜在应用 |
| Identification and potential application of behaviorally active attractant compounds from protein baits for Bactrocera dorsalis |
| 投稿时间：2025-01-21 修订日期：2025-03-31 |
| DOI：10.16688/j.zwbh.2025040 |
| 中文关键词: 蛋白饵剂 橘小实蝇 雌虫引诱剂 |
| 英文关键词:protein bait Bactrocera dorsalis female attractant |
| 基金项目:深圳市科技计划(KCXFZ20240903093859009, KQT20180411143628272)；深圳市大鹏新区科技创新和产业发展专项资金(PT202101-02) |
| 作者 | 单位 | E-mail | | 茹川鉴1, 许光伟3, 王 琳3, 严善春1*, 刘 伟2*, 王桂荣2 | 1. 东北林业大学林学院, 森林生态系统可持续经营教育部重点实验室, 哈尔滨 150040
2. 中国农业科学院(深圳)农业基因组研究所, 岭南现代农业科学与技术广东省实验室深圳分中心, 农业农村部合成生物重点实验室, 深圳 518120
3. 中捷四方(深圳)生物科技有限公司, 深圳 518120 | 严善春yanshanchun@126.com；刘伟liuwei11@cass.cn |
|
| 摘要点击次数: 27 |
| 全文下载次数: 29 |
| 中文摘要: |
| 橘小实蝇Bactrocera dorsalis是全球重要的果蔬害虫, 对柑橘、芒果、番石榴和桃等水果造成严重损失。触杀类化学农药对其防控效果有限, 需频繁施用, 导致农药残留风险增加并加速抗药性发展。在此背景下, 以引诱剂为基础的绿色防控技术, 因其能够减少对化学农药的依赖、降低农药残留风险, 并具有环境友好的特点, 展现出显著的应用潜力。目前, 橘小实蝇引诱剂主要包括雄性引诱剂和蛋白饵剂, 其中蛋白饵剂因能够诱集雌虫, 具有较好的应用前景。蛋白饵剂是一类蛋白源发酵物, 其成分复杂且稳定性不足。因此, 本研究基于具备一定田间引诱能力的蛋白饵剂, 利用分析化学和行为学研究对其行为学活性组分进行了鉴定, 开发新型实蝇引诱剂配方, 并测试了不同配方的竞争或增效效果。研究结果表明, 原始饵剂在暴露空气0、3 d和7 d后均对雌雄成虫有诱集效果, 以暴露0 d的诱集能力最强。进一步利用固相微萃取技术对暴露0 d的原始饵剂顶空挥发物进行收集, 并通过高分辨气相色谱质谱联用仪进行了3次鉴定。筛选出峰面积占比前五且含量超过1%的物质, 最终得到2种候选配方。行为学测定显示, 2种配方均对雌雄成虫有诱集作用, 配方1效果更强。当去掉高含量物质反式-3-己烯酸后, 配方1的诱集能力显著下降, 表明其为主效物质; 去掉其他4种低含量物质时, 仅雌虫诱集量略降, 说明其对反式-3-己烯酸的诱集作用起一定增效效果。反式-3-己烯酸在0.1 mg剂量下对雌雄虫已有诱集作用, 且随着剂量增加效果增强。本研究进一步探讨了配方1与原始蛋白饵剂的竞争与协同作用。在1 mg下, 配方1诱集能力低于原始蛋白饵剂; 在5 mg和10 mg时, 对雄虫诱集能力相当, 对雌虫诱集能力略低, 整体未能超越原始蛋白饵剂。但将1 mg配方1添加至蛋白饵剂后, 显著提升了蛋白饵剂的诱集能力。上述结果为橘小实蝇新型引诱剂的开发提供了科学依据, 并为未来发展针对雌虫的诱杀技术奠定了基础。 |
| 英文摘要: |
| Bactrocera dorsalisis (Hendel) is a highly destructive pest of fruits and vegetables worldwide, infesting citrus, mango, guava, peach, and other crops. Conventional contact insecticides provide limited control and require repeated applications, 〖HJ2.2mm〗increasing residue risks and accelerating resistance development. Environmentally friendly lure-based control strategies have thus become a promising alternative. Among these, protein baits are particularly effective for attracting female B.dorsalis, yet their complex fermentation-derived composition and limited stability restrict consistent performance. In this study, a commercial protein bait with demonstrated field efficacy was analyzed to identify its behaviorally active attractant compounds using analytical chemistry and behavioral bioassays. Results showed that fresh bait (0 day after exposure) exhibited the strongest attraction to both sexes, followed by 3-day and 7-day aged baits. Headspace volatiles were collected via solid-phase microextraction (SPME) and analyzed using gas chromatography-mass spectrometry (GC-MS), revealing five major compounds (>1% relative abundance). Two candidate formulations were developed accordingly. Behavioral assays indicated that both formulations were attractive to males and females, with Formulation 1 being the most effective. Removal of the high-abundance compound trans-3-hexenoic acid significantly reduced attraction, confirming it as the primary active attractant, while exclusion of the other four low-abundance compounds slightly reduced female attraction, suggesting synergistic effects. Trans-3-hexenoic acid alone elicited dose-dependent attraction at concentrations ≥0.1 mg. At 1 mg, Formulation 1 was less attractive than the original bait, but at 5 mg and 10 mg, male attraction was comparable and female attraction slightly lower. Interestingly, supplementation of 1 mg Formulation 1 to the original protein bait significantly enhanced overall attractiveness. These findings identify trans-3-hexenoic acid as a key active component of protein baits and provide a scientific foundation for developing new attractant formulations targeting female B.dorsalis, facilitating the advancement of sustainable attract-and-kill strategies. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
