| 韩珍玉1, 郑慧盈1, 林雨晴1, 王快快1, 缪卫国1, 吴 薇1, 2, 林春花1, 2*.芒果炭疽病菌纳米材料抑菌剂的室内筛选[J].植物保护,2026,(1):185-194. |
| 芒果炭疽病菌纳米材料抑菌剂的室内筛选 |
| Laboratory screening of nanomaterial-based antifungal agents against the pathogen of mango anthracnose |
| 投稿时间：2025-02-26 修订日期：2025-05-12 |
| DOI：10.16688/j.zwbh.2025086 |
| 中文关键词: 芒果 炭疽病菌 纳米抑菌剂 抑菌活性 |
| 英文关键词:mango Colletotrichum nanomaterial-based antifungal agents antifungal activity |
| 基金项目:海南省芒果产业技术体系专项资金（HNARS-07-G03） |
| 作者 | 单位 | E-mail | | 韩珍玉1, 郑慧盈1, 林雨晴1, 王快快1, 缪卫国1, 吴 薇1, 2, 林春花1, 2* | 1. 海南大学热带农林学院, 热带农林生物灾害绿色防控教育部重点实验室, 儋州 571737
2. 海南大学三亚南繁研究院, 三亚 572025 | lin3286320@hainanu.edu.cn |
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| 中文摘要: |
| 纳米材料在提高作物产量和控制病虫害方面具有极大的应用潜力。炭疽病是芒果重要的采前和采后病害, 对芒果产业危害严重。本研究以芒果亚洲炭疽菌Colletotrichum asianum C.a 02-3菌株为研究对象, 采用菌丝生长速率法测定了10种商品化纳米材料对其的抑菌效果, 并选择抑菌效果最好的3种纳米材料, 测定它们对C.a 02-3菌株分生孢子萌发、附着胞产生和致病力的影响, 并测定了它们对芒果4种常见炭疽菌的抑菌效果。结果表明:10种纳米材料对C.a 02-3菌株均有一定抑菌效果, 其中以MgO NPs、ZnO NPs、Cu NPs-1的抑制活性最高, 800 μg/mL MgO NPs与300 μg/mL Cu NPs-1对亚洲炭疽菌有致死效果; 3种纳米材料均可抑制C.a 02-3分生孢子萌发和附着胞形成, 其中以Cu NPs-1和ZnO NPs抑制效果最佳, 三者对芒果炭疽病的预防效果优于治疗效果; 3种纳米材料对芒果4种常见炭疽病菌均具有较好抑制效果, 其中以Cu NPs-1的抑制效果最好, EC50为142.245~187.483 μg/mL。本研究结果为纳米材料在芒果炭疽病等植物病害的防控应用提供了依据。 |
| 英文摘要: |
| Nanomaterials possess tremendous potential for enhancing crop yield and controlling plant diseases and pests. Anthracnose, a critical pre- and post-harvest disease of mango (Mangifera indica), poses a severe threat to mango industry. In this study, the antifungal efficacy of ten commercial nanomaterials against Colletotrichum asianum strain C.a 02-3 was evaluated using the mycelial growth rate method. The three nanomaterials exhibiting the strongest inhibitory effects (MgO nanoparticles (NPs), ZnO NPs, and Cu NPs-1) were further investigated for their impacts on conidial germination, appressorium formation, and pathogenicity of C.a 02-3, as well as their inhibitory activity against four common Colletotrichum species causing mango anthracnose. Results demonstrated that all ten nanomaterials inhibited the growth of C.a 02-3 to varying degrees, with MgO NPs, ZnO NPs, and Cu NPs-1 showing the highest antifungal activity. Specifically, 800 μg/mL MgO NPs and 300 μg/mL Cu NPs-1 exhibited lethal effects on C.asianum. The three selected nanomaterials significantly suppressed conidial germination and appressorium formation, among which Cu NPs-1 and ZnO NPs were most effective. Their preventive efficacy against mango anthracnose surpassed their therapeutic effects. Furthermore, all three nanomaterials demonstrated substantial antifungal activity against four prevalent Colletotrichum species infecting mango, with Cu NPs-1 exhibiting the highest efficacy (EC50 values ranging from 142.245 to 187.483 μg/mL). These findings provide a scientific basis for applying nanomaterials in the management of mango anthracnose and other plant diseases. |
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