| 李洁彩1, 2#, 何 伟1#, 黄 欣1, 罗文芳1, 周军辉1, 张 祥1, 王 俊3*, 许建军1*.侵染番茄的番茄褐色皱纹果病毒新疆分离物的分子鉴定及基因组序列分析[J].植物保护,2026,(1):121-127. |
| 侵染番茄的番茄褐色皱纹果病毒新疆分离物的分子鉴定及基因组序列分析 |
| Molecular identification and whole-genome sequence analysis of tomato brown rugose fruit virus infecting tomato in Xinjiang |
| 投稿时间：2024-12-19 修订日期：2025-02-14 |
| DOI：10.16688/j.zwbh.2024667 |
| 中文关键词: 番茄褐色皱纹果病毒 新疆喀什 番茄 分子鉴定 全基因组分析 |
| 英文关键词:tomato brown rugose fruit virus Kashgar, Xinjiang tomato molecular identification whole-genome analysis |
| 基金项目:自治区公益性科研院所基本科研业务费（KY2024021）；新疆维吾尔自治区重大科技专项（2022A02005-3） |
| 作者 | 单位 | E-mail | | 李洁彩1, 2#, 何 伟1#, 黄 欣1, 罗文芳1, 周军辉1, 张 祥1, 王 俊3*, 许建军1* | 1. 新疆维吾尔自治区农业科学院植物保护研究所, 农业农村部西北荒漠作物有害生物综合治理重点实验室新疆农业生物安全重点实验室, 乌鲁木齐 830091
2. 新疆农业大学, 乌鲁木齐 830052
3. 新疆维吾尔自治区植物保护站, 乌鲁木齐 844099 | 王俊56675148@qq.com; 许建军xjj72@163.com |
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| 中文摘要: |
| 番茄褐色皱纹果病毒(tomato brown rugose fruit virus, ToBRFV)可以侵染包括番茄在内的多种茄科作物, 对番茄的产量和品质造成显著影响。本研究利用小RNA测序技术, 从新疆喀什地区叶城县的番茄病样中检测到ToBRFV, 将该分离物命名为ToBRFV-XJ, 该病毒与南方番茄病毒(southern tomato virus, STV)、番茄花叶病毒(tomato mosaic virus, ToMV)复合侵染番茄。通过设计的特异性引物成功扩增了ToBRFV-XJ的基因组序列。基因组全长6 264 nt, 包括4个开放阅读框(ORFs), 分别编码4个蛋白。系统发育分析表明, ToBRFV-XJ与山东(登录号: MT018320.1)和辽宁(登录号: OR437354.1)分离物具有较近的亲缘关系。本研究首次监测到了ToBRFV在新疆设施番茄上发生危害, 对控制ToBRFV在新疆的扩散具有重要意义。 |
| 英文摘要: |
| Tomato brown rugose fruit virus (ToBRFV) is newly emerging virus that infects various Solanaceous crops, particularly tomato (Solanum lycopersicum), causing substantial losses in both yield and quality. In this study, ToBRFV was detected in diseased tomato samples collected from Yecheng county, Kashgar prefecture, Xinjiang, by using small RNA sequencing technology. The isolate was designated ToBRFV-XJ, and was found to co-infect tomato plants together with southern tomato virus (STV) and tomato mosaic virus (ToMV). The complete genome sequence of ToBRFV-XJ, comprising 6 264 nucleotides and containing four open reading frames (ORFs) that encode four proteins, was successfully amplified using specifically designed primers. Phylogenetic analysis indicated that ToBRFV-XJ is closely related to the Shandong (GenBank accession no. MT018320.1) and Liaoning (GenBank accession no. OR437354.1) isolates. This study represents the first report of ToBRFV infecting greenhouse tomatoes in Xinjiang, and provides important insights for the monitoring and prevention of ToBRFV spread in this region. |
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