| 李南楠1, 2#, 张亚封1, 2#, 李启云1, 2, 路 杨1, 张正坤1*, 隋 丽1*.球孢白僵菌内生定殖诱导番茄抗立枯病[J].植物保护,2026,(1):103-112. |
| 球孢白僵菌内生定殖诱导番茄抗立枯病 |
| Endophytic colonization of Beauveria bassiana induces resistance to damping-off disease in tomato |
| 投稿时间：2025-01-08 修订日期：2025-02-24 |
| DOI：10.16688/j.zwbh.2025012 |
| 中文关键词: 球孢白僵菌 立枯丝核菌 土传病害 植物保护 |
| 英文关键词:Beauveria bassiana Rhizoctonia solani soil-borne diseases plant protection |
| 基金项目:国家自然科学基金（32271683）；吉林省农业科技创新工程（CXGC2024RCY015，CXGC2024JJ011) ；三江实验室科技创新项目（SJ2025008-1） |
| 作者 | 单位 | E-mail | | 李南楠1, 2#, 张亚封1, 2#, 李启云1, 2, 路 杨1, 张正坤1*, 隋 丽1* | 1. 吉林省农业科学院植物保护研究所, 吉林省农业微生物重点实验室, 农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100
2. 吉林农业大学, 长春 130118 | 张正坤zhangzhengkun1980@126.com；隋丽suiyaoyi@163.com |
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| 中文摘要: |
| 为探究球孢白僵菌Beauveria bassiana在番茄Solanum lycopersicum植株体内的定殖效果及其对土传病害——番茄立枯病的防治效果和作用机理, 本文测定了球孢白僵菌不同接种方式(浸种和灌根)对立枯丝核菌Rhizoctonia solani胁迫下番茄植株生长的影响; 通过检测盆栽和大田条件下球孢白僵菌的定殖能力, 番茄立枯病发病率、病情指数，防治效果以及立枯丝核菌相对含量, 明确球孢白僵菌对番茄立枯病抗性的影响。结果表明, 两种处理方式接种球孢白僵菌, 球孢白僵菌均能够有效地在植物体内定殖, 并在定殖后迅速萌发。球孢白僵菌的定殖能显著提升番茄植株的叶绿素含量和植株高度, 显著降低番茄发病率和病情指数, 对立枯丝核菌在植株体内的扩散具有显著的抑制效果; 在立枯丝核菌胁迫下, 球孢白僵菌在番茄叶片和根部内相对含量显著增加, 病原菌相对含量降低, 番茄植株的抗病性提高。本研究明确了球孢白僵菌能够在番茄植株内定殖, 且在立枯丝核菌胁迫下, 其定殖不仅对番茄植株的生长具有促进作用, 还可以提高植株对病原菌的抗性。该研究为探索虫生真菌的植物内生性及其对寄主植物的正向调控作用提供理论依据。 |
| 英文摘要: |
| To explore the endophytic colonization of Beauveria bassiana in tomato (Solanum lycopersicum) plants and its effects and mechanisms in controlling soil-borne pathogen Rhizoctonia solani, the influence of different inoculation methods of B.bassiana on tomato growth under R.solani stress were evaluated. The colonization ability of B.bassiana, disease incidence, control efficacy and relative abundance of R.solani were assessed under pot and field conditions to clarify the role of B.bassiana in inducing resistance to tomato damping-off disease. The results showed that B.bassiana effectively colonized tomato tissues and rapidly germinated after inoculation via seed soaking and root irrigation. Endophytic colonization by B.bassiana significantly increased chlorophyll content and plant height, reduced disease incidence and disease index, and markedly inhibited the spread of R.solani within plants. Under R.solani stress, the relative abundance of B.bassiana in tomato leaves and roots increased, while that of the pathogen decreased, indicating enhanced host resistance. This study demonstrated that B.bassiana can successfully establish endophytic colonization in tomato plants, and promote both growth and disease resistance under pathogen stress, providing a theoretical basis for understanding the plant endophytism of entomopathogenic fungi and their positive regulatory roles in host defense. |
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