| 于 欢1, 汤金荣1, 房风云1, 王文汇1, 张彩虹1, 李国平3, 梁革梅1, 2*.Cry1Ac抗性棉铃虫对转Bt基因玉米的敏感性变化及机制研究[J].植物保护,2026,(1):90-102. |
| Cry1Ac抗性棉铃虫对转Bt基因玉米的敏感性变化及机制研究 |
| Sensitivity changes and mechanisms of Cry1Ac-resistant cotton bollworm to transgenic Bt maize |
| 投稿时间：2025-01-24 修订日期：2025-04-02 |
| DOI：10.16688/j.zwbh.2025050 |
| 中文关键词: 棉铃虫 转基因抗虫玉米 Bt 交互抗性 机制 |
| 英文关键词:Helicoverpa armigera transgenic insect-resistant maize Bacillus thuringiensis cross resistance mechanism |
| 基金项目:农业生物育种国家科技重大专项(2022ZD04021); 河南省农业科学院基础性科研工作项目(2023SWYZ01) |
| 作者 | 单位 | E-mail | | 于 欢1, 汤金荣1, 房风云1, 王文汇1, 张彩虹1, 李国平3, 梁革梅1, 2* | 1. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193
2. 中国农业科学院西部农业研究中心, 昌吉 831100
3. 河南省农业科学院植物保护研究所, 郑州 450002 | gmliang@ippcaas.cn |
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| 中文摘要: |
| 转基因抗虫作物可以有效防治靶标害虫, 但随着种植时间的延长, 害虫产生抗性的风险也会增加, 因此提前评估害虫的抗性风险, 制定合理的抗性治理策略, 对转基因作物的产业化应用至关重要。本文采用饲料混合法测定了Cry1Ac抗性棉铃虫Helicoverpa armigera品系(LF10、LF30、LF60、LF120)对3种转Bt基因玉米(‘DBN9936’‘DBN3601T’‘瑞丰125’)的敏感性变化, 并利用实时荧光定量技术比较了取食常规玉米和转基因Bt玉米后棉铃虫Bt受体基因转录水平的差异。结果表明, 与敏感品系相比, Cry1Ac抗性品系棉铃虫对3种转基因玉米的敏感性下降, 且对Cry1Ac抗性水平越高的品系，对转基因玉米的敏感性越低。与取食常规玉米相比, 取食转基因玉米会影响棉铃虫Bt受体基因的表达, 3种抗虫玉米对相同受体基因的转录水平，对不同品系棉铃虫受体基因转录水平的影响均存在较大差异, 总体趋势表现为取食转基因Bt玉米后不同品系棉铃虫体内ABCC2的转录水平降低。取食同种转基因Bt玉米时, 与敏感棉铃虫相比, 抗性棉铃虫的ABCC2和CAD转录水平也显著降低。因此, 棉铃虫取食抗虫玉米时, 受Bt蛋白胁迫, ABCC2可能在应急反应中起主要作用; Cry1Ac抗性棉铃虫对3种转基因Bt玉米(表达蛋白中含有Cry1Ab)产生明显的交互抗性, 抗性棉铃虫ABCC2和CAD基因转录水平的显著降低可能是交互抗性产生的主要原因。 |
| 英文摘要: |
| Transgenic insect-resistant crops can effectively control target pests, however, with prolonged cultivation, the risk of pests developing resistance increases, thus, assessing pest resistance risk in advance and developing rational resistance management strategies are crucial for the sustainable application of transgenic crops. In this study, the sensitivity changes of Cry1Ac-resistant Helicoverpa armigera strains (LF10, LF30, LF60, LF120) feeding on three transgenic Bt maize cultivars (‘DBN9936’ ‘DBN3601T’ ‘Ruifeng 125’) were determined using the diet incorporation bioassay. The transcriptional levels of major Bt receptor genes were compared between larvae fed on conventional maize and those fed on Bt maize using quantitative real-time PCR (RT-qPCR). The results indicated that the Cry1Ac-resistant strains exhibited reduced sensitivity to all three Bt maize cultivars compared with the susceptible strain, and the sensitivity declined with increasing Cry1Ac resistance level in the resistant strains. Feeding on Bt maize altered the expression of Bt receptor genes in H.armigera, with varying effects among maize cultivars and between different insect strains. Overall, the transcription of ABCC2 was down-regulated after fed on transgenic Bt maize. When feeding on the same Bt maize, resistant H.armigera showed significantly lower transcription levels of ABCC2 and CAD than the susceptible strain. These results suggest that ABCC2 may play a major role in the stress response of H.armigera under Bt protein exposure. The Cry1Ac-resistant H.armigera showed obvious cross-resistance to the three Bt maize cultivars (which express Cry1Ab proteins), and the significant down-regulation of ABCC2 and CAD in resistant strains may be the main molecular basis for the cross-resistance. |
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