| 邱维湘1, 张朝阳1, 杨欣朋1, 郑平钰2, 李晓刚1*.基于纤维素纳米晶与表面活性剂的Pickering 乳液增强对柑橘叶片的亲和性[J].植物保护,2026,(1):47-56. |
| 基于纤维素纳米晶与表面活性剂的Pickering 乳液增强对柑橘叶片的亲和性 |
| Enhanced adhesion of Pickering emulsions stabilized by cellulose nanocrystals and surfactants to citrus leaves |
| 投稿时间：2024-12-30 修订日期：2025-01-19 |
| DOI：10.16688/j.zwbh.2024690 |
| 中文关键词: 纤维素纳米晶 Pickering乳液 香芹酚 叶片亲和性 生物活性 |
| 英文关键词:cellulose nanocrystal Pickering emulsion carvacrol leaf affinity bioactivity |
| 基金项目:国家自然科学基金（32302335）；宁夏回族自治区重点研究计划（2024BBF02025） |
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| 中文摘要: |
| 纤维素纳米晶(cellulose nanocrystal, CNC)基Pickering乳液(PE)因高稳定性和生物相容性, 在农业防治领域具有应用前景。本研究选择香芹酚(carvacrol, CAR)作为抑菌活性成分, 制备基于CNC和表面活性剂的载药Pickering乳液(CAR@PE), 并探究其对柑橘黄单胞菌柑橘亚种Xanthomonas citri subsp. citri (Xcc)的生物活性。通过透射电子显微镜(TEM)和X射线衍射(XRD)表征CNC, 采用乳析指数(creaming index, CI)筛选CNC和表面活性剂的最佳组合, 并测定CAR@PE的环境稳定性、流变性、叶片界面行为等性质。结果表明, 本研究使用的CNC材料是一种纳米级棒状晶体, 晶型类别为Ⅰ型纤维素。含有CNC和表面活性剂SK-551的CAR@PE的CI为0, 稳定不分层。在不同温度、pH和离子强度下具有良好的稳定性。CAR@PE为非牛顿液体, 具有剪切稀化现象, 高频下产生相变。此外，CAR@PE具有较低的表面张力（28.70 mN/m）和接触角（27.94°）, 在柑橘叶片表面具有良好铺展效果, 其沉积率为24.94%, 对Xcc的EC50为24.28 mg/L。基于上述结果, 本研究拓展了Pickering乳液在农业病害防控中的应用, 探索了CAR对Xcc的活性, 为防控柑橘溃疡病提供了一种新途径。 |
| 英文摘要: |
| Cellulose nanocrystal (CNC)-based Pickering emulsions (PE) possess great potential for agricultural pest management owing to their high stability and biocompatibility. In this study, carvacrol (CAR) was selected as the antibacterial active ingredient to prepare a CNC/surfactant synergistically stabilized CAR-loaded Pickering emulsion (CAR@PE), and its biological activity against Xanthomonas citri subsp. citri (Xcc) was evaluated. CNC was characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The optimal formulation was screened out using the creaming index (CI), and the emulsion properties were examined in terms of environmental stability, rheological property, and leaf surface interaction. The results showed that the CNC used was nanoscale rod-like crystals belonging to cellulose type I. The CAR@PE containing surfactant SK-551 and CNC exhibited a CI of 0, indicating excellent stability without phase separation. The emulsion maintained good stability under various temperature, pH and ionic strength conditions. CAR@PE behaved as a non-Newtonian fluid with shear-thinning characteristics and underwent phase transitions at high frequencies. Its surface tension was 28.70 mN/m, and the contact angle was 27.94°, demonstrating superior spreading performance and a deposition rate of 24.94% on citrus leaves. The EC50 value against Xcc was 24.28 mg/L. These findings broaden the application of Pickering emulsions in agricultural disease prevention and control, reveal the bioactivity of CAR against Xcc, and provide a novel strategy for controlling citrus canker disease. |
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