| 黄维依1, 乔 开1, 李学美1, 周宇杰1, 苏 帆2, 林木森3*, 蔡 红1*.喜树丛枝植原体基因组组装及功能分析[J].植物保护,2026,(1):27-38. |
| 喜树丛枝植原体基因组组装及功能分析 |
| Genome assembly and functional analysis of Camptotheca acuminata witches’-broom phytoplasma |
| 投稿时间：2025-01-09 修订日期：2025-03-24 |
| DOI：10.16688/j.zwbh.2025018 |
| 中文关键词: 喜树丛枝植原体 基因组草图 代谢途径 分泌蛋白 |
| 英文关键词:Camptotheca acuminata witches’-broom phytoplasma genome draft metabolic pathways secreted proteins |
| 基金项目:国家自然科学基金(31960535) |
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| 摘要点击次数: 28 |
| 全文下载次数: 30 |
| 中文摘要: |
| 为阐明喜树丛枝植原体的基因组结构, 采用高通量测序技术和Phytoassembly pipeline组装方法, 从宿主基因组信息中过滤组装得到该植原体基因组草图。该基因组全长为608 891 bp, 包含642个编码序列、32个tRNA基因和2个rRNA操纵子。注释分析显示, 该植原体缺少与氨基酸和脂肪酸生物合成、三羧酸循环、氧化磷酸化、磷酸戊糖途径相关的部分基因, 但其糖酵解和丙酮酸氧化降解途径完整, 表明其可能主要依赖这两条途径获取能量。此外, 基因组中富含ABC转运蛋白基因, 参与亚精胺/腐胺、二肽/寡肽、钴、锰、锌及氨基酸等物质的转运。值得注意的是, 该基因组拥有苹果酸转运蛋白和苹果酸脱氢酶相关的编码基因, 意味着该植原体可能利用寄主苹果酸作为ATP产生的碳源。该基因组中存在与翠菊黄化植原体分泌蛋白SAP11AY-WB和SAP05AY-WB同源的分泌蛋白, 推测这些蛋白可能与诱导喜树丛枝症状相关。系统发育分析表明, 该植原体与16Sr Ⅷ组的Candidatus Phytoplasma luffae关系最为密切。本研究首次报告了喜树丛枝植原体基因组序列, 为植原体致病机制研究和难培养微生物的基因组测序提供了参考。 |
| 英文摘要: |
| To elucidate the genome structure of the Camptotheca acuminata witches’-broom phytoplasma, high-throughput sequencing combined with the Phytoassembly pipeline was employed to assemble a draft genome filtered from host genomic data. The assembled genome is 608 891 bp in length and contains 642 coding sequences, 32 tRNA genes, and two rRNA operons. Annotation analysis revealed that this phytoplasma lacks several genes related to amino acid and fatty acid biosynthesis, the tricarboxylic acid cycle, oxidative phosphorylation, and the pentose phosphate pathway. However, genes for glycolysis and pyruvate oxidation are intact, suggesting that the phytoplasma mainly depends on these two pathways for energy production. Additionally, the genome is enriched in genes encoding ATP-binding cassette (ABC)transporters, which are predicted to mediate the transport of spermidine/putrescine, dipeptides/oligopeptides, cobalt, manganese, zinc, and amino acids. Notably, the presence of genes encoding malate transporter and malate dehydrogenase indicates that this phytoplasma may utilize host-derived malate as a carbon source for ATP synthesis. Two secreted proteins homologous to the aster yellows phytoplasma effectors SAP11AY-WB and SAP05AY-WB were also identified, suggesting their possible involvement in the induction witches’-broom symptoms. Phylogenetic analysis showed that this phytoplasma is most closely related to ‘Candidatus Phytoplasma luffae’ within the 16Sr Ⅷ group. This study, for the first time, reports the genome sequence of the Camptotheca acuminata witches’-broom phytoplasma, providing a valuable reference for understanding phytoplasma pathogenic mechanisms and advancing genomic research on obligate, difficult-to-culture microorganisms. |
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