| 高维芳1, 2#, 李 雪2#, 赵程通2, 姜 露2, 贾明轩2, 周雪平2, 3*, 李 星1*, 李方方2*.大白菜水杨酸通路基因BraNPR1、BraTGA3参与调控芜菁花叶病毒侵染[J].植物保护,2026,(1):17-26. |
| 大白菜水杨酸通路基因BraNPR1、BraTGA3参与调控芜菁花叶病毒侵染 |
| BraNPR1 and BraTGA3 from the salicylic acid signaling pathway in Chinese cabbage mediate resistance against turnip mosaic virus |
| 投稿时间：2025-01-13 修订日期：2025-04-29 |
| DOI：10.16688/j.zwbh.2025025 |
| 中文关键词: 大白菜 芜菁花叶病毒 NPR1 TGA3 相互作用机制 |
| 英文关键词:Chinese cabbage turnip mosaic virus NPR1 TGA3 interaction mechanism |
| 基金项目:国家自然科学基金(32172385) |
| 作者 | 单位 | E-mail | | 高维芳1, 2#, 李 雪2#, 赵程通2, 姜 露2, 贾明轩2, 周雪平2, 3*, 李 星1*, 李方方2* | 1. 河北农业大学植物保护学院, 保定 071000
2. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193
3. 浙江大学生物技术研究所, 杭州 310058 | 周雪平zzhou@zju.edu.cn; 李星lxkzh@163.com; 李方方ffli@ippcaas.cn |
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| 中文摘要: |
| 水杨酸是重要的植物激素与信号分子, 通过调控免疫相关基因NPR1以及转录因子TGA等的表达, 调节对入侵病原微生物的抗性。为了探讨大白菜水杨酸通路相关基因在植物与病毒互作中的功能, 以芜菁花叶病毒(turnip mosaic virus, TuMV)敏感大白菜品种‘良庆’与本氏烟Nicotiana benthamiana为试验材料, 研究NPR1、TGA3与TuMV之间的相互作用机制。通过同源性比对与进化关系分析, 在大白菜基因组中鉴定到水杨酸信号通路相关基因BraNPR1与TGA基因家族的8个BraTGAs, RT-qPCR检测发现它们均能够响应TuMV的侵染。通过亚细胞定位分析、酵母双杂交以及双分子荧光互补试验, 证明了BraNPR1能够与BraTGA3在细胞核中互作。进一步将TuMV分别与BraNPR1、BraTGA3共浸润本氏烟发现, 过表达BraNPR1和BraTGA3促进病程相关蛋白PR1基因的表达, 抑制了病毒侵染。综上, 大白菜水杨酸通路相关基因BraNPR1与BraTGA3相互作用响应病毒侵染, 参与对植物病毒的抗性。 |
| 英文摘要: |
| Salicylic acid (SA) functioning as a acrucial phytohormone and signaling molecule, which regulates plant immune responses by modulating the expression of defense-related genes such as NPR1, TGA transcription factors. To elucidate the roles of salicylic acid pathway genes in the interaction between Chinese cabbage and viruses, the turnip mosaic virus (TuMV)-susceptible cultivar ‘Liangqing’ and Nicotiana benthamiana were used as plant materials. Through sequence homology and phylogenetic analysis, BraNPR1 and eight BraTGAs in TGA family members were identified in the genome of Chinese cabbage. RT-qPCR analysis revealed that these genes were transcriptionally responsive to TuMV infection. Subcellular localization, yeast two-hybrid, and bimolecular fluorescence complementation assays confirmed that BraNPR1 interacts with BraTGA3 in the nucleus. Additionally, co-infiltration assays in N.benthamiana revealed that overexpression of BraNPR1 and BraTGA3 upregulated the pathogenesis-related protein 1 gene (PR1) and inhibited viral accumulation. In conclusion, these findings demonstrate that BraNPR1 and BraTGA3 function cooperatively in the salicylic acid signaling pathway to mediate antiviral defense against TuMV in Chinese cabbage. |
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