| 王 敏1, 王新溪1, 王圣印2*.橘小实蝇对甲氨基阿维菌素苯甲酸盐的抗性机制及对其他药剂的交互抗性[J].植物保护,2023,49(1):278-284. |
| 橘小实蝇对甲氨基阿维菌素苯甲酸盐的抗性机制及对其他药剂的交互抗性 |
| Resistance mechanisms of Bactrocera dorsalis population to emamectin benzoate and its cross-resistance to other insecticides |
| 投稿时间：2021-10-24 修订日期：2022-01-20 |
| DOI：10.16688/j.zwbh.2021586 |
| 中文关键词: 橘小实蝇 甲维盐 多杀霉素 虫螨腈 交互抗性 抗性机制 |
| 英文关键词:Bactrocera dorsalis emamectin benzoate spinosad chlorfenapyr cross-resistance resistance mechanism |
| 基金项目:国家自然科学基金（31701848） |
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| 中文摘要: |
| 入侵害虫橘小实蝇严重为害蔬菜、花卉、水果等经济作物, 目前在田间常使用杀虫剂进行防治。为探索甲氨基阿维菌素苯甲酸盐(甲维盐)防控橘小实蝇的抗性风险, 采用药膜法测定了橘小实蝇抗甲维盐种群(EB)对8种杀虫剂的交互抗性水平, 并测定了橘小实蝇EB种群、金华田间种群(JH)和敏感种群(S)的解毒酶活性。历经33代筛选, 橘小实蝇EB种群对甲维盐产生了43.4倍的中等水平抗性, 对阿维菌素存在中等水平交互抗性, 对吡虫啉、辛硫磷、马拉硫磷存在低水平交互抗性, 对噻虫胺、高效氯氟氰菊酯、多杀霉素和虫螨腈未表现出交互抗性。胡椒基丁醚(PBO)、磷酸三苯酯(TPP)和顺丁烯二酸二乙酯(DEM)均可显著提高甲维盐对橘小实蝇EB种群、JH种群和S种群的室内毒力。橘小实蝇EB种群细胞色素P450(3.9倍)和b5含量(3.3倍)、O -脱甲基酶活性(4.2倍)、谷胱甘肽S-转移酶活性(2.7倍)、羧酸酯酶活性(3.2倍)显著高于S种群。多功能氧化酶、谷胱甘肽S-转移酶、羧酸酯酶活性显著增强可能是橘小实蝇对甲维盐产生高水平抗性的重要机制。 |
| 英文摘要: |
| Bactrocera dorsalis is a destructive quarantine pest of vegetables, flowers and fruits, and insecticides are often used to control this pest in the field. In order to explore the resistance risk, the cross resistance to eight insecticides was investigated using the method of drug film in the glass tube. In addition, the biochemical resistance mechanism of the resistant population (EB), Jinhua field population (JH) and susceptible population (S) of B.dorsalis were also studied. After selecting for 33 generations, B.dorsalis showed a medium level of resistance to emamectin benzoate (43.4-fold). The results showed that the EB population had a medium level of cross-resistance to abamectin, and a low level of cross-resistance to imidacloprid, phoxim and malathion, but no cross-resistance to clothianidin, lambda-cyhalothrin, spinosad and chlorfenapyr. All of the synergists, including piperonyl butoxide (PBO), triphenyl phosphate (TPP) and diethyl maleate (DEM), had a significant synergistic effect on emamectin benzoate in killing the EB, JH and S populations. Compared to the S population, the activities of mixed-function oxidases P450 (3.9-fold), b5 (3.3-fold) and O -demethylase (4.2-fold), glutathione S -transferases (2.7-fold) and carboxylesterase (3.2-fold) increased significantly in the EB population. The results indicated that the increased mixed-function oxidases, glutathione S -transferases and carboxylesterase might play an important role in the resistance of B.dorsalis to emamectin benzoate. |
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