| 孔卫青, 凌 君, 杨金宏*.畸形桑叶中病毒的鉴定及其小RNA特征分析[J].植物保护,2022,48(5):251-255. |
| 畸形桑叶中病毒的鉴定及其小RNA特征分析 |
| Identification and analysis of viruses and small RNA in deformed mulberry leaves |
| 投稿时间：2021-09-08 修订日期：2021-11-25 |
| DOI：10.16688/j.zwbh.2021498 |
| 中文关键词: 畸形桑叶 病毒 复合侵染 小RNA |
| 英文关键词:deformed mulberry leaf virus mixed infection small RNA |
| 基金项目:陕西省重点研发计划(2018ZDXM-NY-057); 陕西高校青年创新团队项目(2020AYSC01) |
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| 中文摘要: |
| 从安康市桑园采集了68份畸形桑树叶片样品, 利用PCR扩增检测鉴定其中存在4种病毒, 分别是桑花叶型萎缩病相关病毒(mulberry mosaic dwarf-associated virus, MMDaV)?桑花叶卷叶病相关病毒(mulberry mosaic leaf roll associated virus, MMLRaV)?桑花叶萎缩类病毒(mulberry mosaic dwarf viroid, MMDVd)和桑潜隐病毒(mulberry cryptic virus, MuCV), 存在复合侵染?对4种病毒复合侵染桑叶进行小RNA深度测序和分析, 结果显示其中的sRNA来自MMDaV?MMLRaV?MMDVd?MuCV 4种病毒, 与RT-PCR检测结果一致?进一步分析sRNA长度和5′-末端序列, MMDaV sRNA长度以24 nt最多, 其他病毒sRNA主要为21 nt?MMDaV基因组链sRNA 5′-末端以A和T为主, MuCV以C最多, MMLRaV和MMDVd一致, 均以T最多? |
| 英文摘要: |
| A total of 68 deformed mulberry leaves were collected from mulberry garden in Ankang city. Four viruses including mulberry mosaic dwarf-associated virus (MMDaV), mulberry mosaic leaf roll associated virus (MMLRaV), mulberry mosaic dwarf viroid (MMDVd) and mulberry cryptic virus (MuCV), and mix-infection were found by RT-PCR amplification in the samples. And then, the small RNA deep-sequencing and analysis of mulberry leaves mix-infected by four viruses were carried out. The results showed that the sRNA was from four viruses of MMDaV, MMLRaV, MMDVd and MuCV, which was in accordance with that from RT-PCR. The analysis of sRNA length showed that the predominated length of MMDaV sRNA was 24 nt, while 21 nt for other three viruses. The results of 5′-terminus nucleotide bias showed that 5′-terminus nucleotide of MMDaV genomic strands sRNA are most A and T, C for MuCV, while MMLRaV and MMDVd are both preferred T. |
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