| 蒋春艳.金龟子绿僵菌对黄曲条跳甲体内保护酶活性的影响[J].植物保护,2022,48(5):129-133. |
| 金龟子绿僵菌对黄曲条跳甲体内保护酶活性的影响 |
| Effects of infection by Metarhizium anisopliae on the activities of protective enzymes in Phyllotreta striolata |
| 投稿时间：2021-07-27 修订日期：2021-09-06 |
| DOI：10.16688/j.zwbh.2021411 |
| 中文关键词: 金龟子绿僵菌 黄曲条跳甲 超氧化物歧化酶 过氧化物酶 过氧化氢酶 |
| 英文关键词:Metarhizium anisopliae Phyllotreta striolata superoxide peroxidase catalase |
| 基金项目:福建省科技厅农业引导性项目(2018N0058) |
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| 中文摘要: |
| 为探索绿僵菌对黄曲条跳甲保护酶活性的影响, 分别用浓度为1×108?2×108?4×108?8×108?1.6×109个/mL的金龟子绿僵菌CQMa421孢子悬浮液处理黄曲条跳甲成虫, 对侵染后黄曲条跳甲体内超氧化物歧化酶(SOD)?过氧化物酶(POD)和过氧化氢酶(CAT)活性进行了研究?结果表明:处理后120 h内, SOD活性先升高后降低, 48 h时SOD活性达最高, 分别为69.5?72.5?74.5?75.9?76.7 U/g, SOD活性与孢子悬浮液浓度呈正相关; 各处理POD活性在处理后72 h时开始上升, 96 h时活性最高, 分别为1 020?1 066.67?1 240?1 086.67?1 166.67 U/g, 分别为对照组的3.33?3.48?4.04?3.54倍和3.80倍, 随后开始下降; CAT活性变化总体趋势为上升—下降—上升—下降; 3种酶活性最后都恢复至对照组水平, 各处理组最大值间差异显著, 且显著高于对照组, 表明3种保护酶在黄曲条跳甲抵御金龟子绿僵菌入侵过程中均做出了应激反应? |
| 英文摘要: |
| To explore the change in the activities of protective enzymes in Phyllotreta striolata infected by Metarhizium anisopliae, 1×108, 2×108, 4×108, 8×108 and 1.6×109 spores/mL spore suspension of M. anisopliae CQMa421 were used to infect the adults of P. striolata, respectively. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of P. striolata were measured after infected by CQMa421. The results showed that, within 120 h after treatment, the activity of SOD of P. striolata was increased at the initial infection stage, but dropped at the later infection stage with increasing time at the same treatment concentration. The activity of SOD reached the highest at 48 h, which was 69.5, 72.5, 74.5, 75.9, 76.7 U/g fresh weight, respectively, and the activity of SOD had a positive correlation with the spore concentration of M. anisopliae CQMa421. The POD activity of treatment groups began to increase at the 72nd-hours and reached the highest at the 96th-hours, which was 1 020, 1 066.67, 1 240, 1 086.67, 1 166.67 U/g fresh weight, respectively, and they were 3.33-, 3.48-, 4.04-, 3.54- and 3.80-folds as much as the control group, but then dropped. The activity of CAT displayed the trend of rise-decrease-rise-decrease within 120 h after treatment. The activities of the three enzymes finally returned to the level equal to the control groups. The maximum values were significantly different among different treatment groups. The activities of SOD, POD and CAT were significantly higher than that of the control group, which indicated that the three protective enzymes responded to the invasion of M. anisopliae. |
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