| 陈星伊, 崔凌霄, 杨成德*, 杨丽萍, 魏立娟, 刘治会.马铃薯块茎病原真菌拮抗菌株筛选及优良拮抗菌株鉴定[J].植物保护,2020,46(2):143-147. |
| 马铃薯块茎病原真菌拮抗菌株筛选及优良拮抗菌株鉴定 |
| Screening and identification of antagonistic bacterial strains against potato tuber pathogenic fungi |
| 投稿时间：2019-01-09 修订日期：2019-02-27 |
| DOI：10.16688/j.zwbh.2019017 |
| 中文关键词: 马铃薯块茎 内生细菌 分离 抑菌功能 鉴定 |
| 英文关键词:potato tuber endophytic bacteria isolation antibacterial function identification |
| 基金项目:国家马铃薯产业技术体系（CARS-10-P18） |
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| 中文摘要: |
| 从马铃薯块茎上分离内生细菌,以块茎病原真菌为指示菌,采用平板对峙法筛选拮抗菌株,并利用形态学特征和 16S rDNA 基因序列分析对优良拮抗菌株进行鉴定。结果表明,从定西马铃薯块茎中共分离得到72株内生细菌,其中19株对5种块茎病原真菌的抑菌率均高于51.87%,特别是内生细菌6-5和5-6分别对马铃薯炭疽病菌Colletotrichum coccodes和马铃薯黑痣病菌Rhizoctonia solani的抑菌率达85.82%和72.18%,且具有固氮和产吲哚乙酸（IAA）功能,其产IAA量分别为3.37 mg/L和19.25 mg/L;根据形态特征及16S rDNA基因序列将菌株5-6鉴定为萎缩芽胞杆菌Bacillus atrophaeus,菌株6-5鉴定为贝莱斯芽胞杆菌Bacillus velezensis。 |
| 英文摘要: |
| In this study, endophytic bacteria were isolated from potato tubers, and tuber pathogenic fungi were used as indicator fungi. Antagonistic strains were screened by using tablet confrontation method, and excellent antagonistic strains were identified by morphological characteristics and 16S rDNA sequence analysis. The results showed that 72 endophytic bacteria were isolated from Dingxi potato tubers, and 19 of them had an inhibition rate of higher than 51.87% against 5 tuber pathogenic fungi; particularly, endophytic bacteria 6-5 and 5-6 had a bacteriostatic rate of 85.82% and 72.18% against Colletotrichum coccodes and Rhizoctonia solani, respectively. They had nitrogen fixation and IAA function, with an IAA yield of 3.37 mg/L and 19.25 mg/L, respectively. Based on morphological characteristics and 16S rDNA sequence, 5-6 and 6-5 were identified as Bacillus atrophaeus and Bacillus velezensis, respectively. |
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