耿涌鑫 1, 2, 杨锦 1, 朱知睿 1, 魏丹峰 1, 陈敏 1*.双条杉天牛幼虫人工饲料的筛选[J].植物保护,2018,44(2):134-139. |
双条杉天牛幼虫人工饲料的筛选 |
Screening for artificial diets for Semanotus bifasciatus |
投稿时间:2017-03-20 修订日期:2017-04-17 |
DOI:10.16688/j.zwbh.2017095 |
中文关键词: 双条杉天牛 人工饲料 正交试验 极差分析 |
英文关键词:Semanotus bifasciatus artificial diet orthogonal analysis range analysis |
基金项目:国家高技术研究发展计划(“863”计划)(2011AA100201) |
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中文摘要: |
双条杉天牛 Semanotus bifasciatus (Motschulsky)是柏科树木最严重的钻蛀性害虫之一。开发双条杉天牛的人工饲料是开展该害虫防治技术研究及生物学相关基础研究的重要条件。本研究在参考了其他天牛人工饲料配方的基础上, 采用正交试验设计方法设计饲料配方, 通过对不同配方饲料对双条杉天牛幼虫的饲养效果进行比较和极差分析, 最终筛选出双条杉天牛幼虫的人工饲料最优组合为:琼脂4.7%、蔗糖3.5%、对-羟基苯甲酸甲酯0.5%、酵母粉3.1%、柏树木粉30%、抗坏血酸0.5%以及水。使用该人工饲料可将双条杉天牛幼虫成功喂养至羽化, 并且蛹羽化率高达81.25%, 总成活率为54.16%。本研究的结果可为天牛科其他天牛人工饲料的开发提供借鉴。 |
英文摘要: |
Semanotus bifasciatus (Coleoptera: Cerambycidae) is one of the most serious stem borers in Cupressaceae trees. Development of artificial diets for S. bifasciatus is is important for further studies on its control strategies and biology. The orthogonal design was used in this research to develop the feed formula based on several feed recipes for long-horned beetles. According to the results of the screening tests, we improved the diet formula and finally obtained an optimal diet formula for S. bifasciatus larvae. The optimal formula was as followed: agar 4.7%, sucrose 3.5%, methyl p -hydroxybenzoate 0.5%, yeast powder 3.1%, Platycladus orientalis wood sawdust 30%, ascorbic acid 0.5% and distilled water 57.7%. This diet could feed the larvae from the initial incubation to pupae successfully, and the pupal emergence rate was as high as 81.25%, with a final survival rate up to 54.16%. The results provide a reference for the development of artificial diets for other long-horned beetles. |
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