| 宣俊好, 张元博, 蒙城功, 彭彪彪, 杨瑞先*.洛阳地区牡丹3种主要叶部病害病原菌的分离与鉴定[J].植物保护,2017,43(6):91-96. |
| 洛阳地区牡丹3种主要叶部病害病原菌的分离与鉴定 |
| Isolation and identification of three main leaf pathogens on tree peony in Luoyang |
| 投稿时间：2017-02-23 修订日期：2017-03-11 |
| DOI： |
| 中文关键词: 牡丹 叶部病害 病原菌 鉴定 |
| 英文关键词:tree peony leaf disease pathogen identification |
| 基金项目:国家自然科学基金(31500008); 河南省科技厅科技攻关项目(152102210328) |
|
| 摘要点击次数: 599 |
| 全文下载次数: 1008 |
| 中文摘要: |
| 为明确引起洛阳牡丹Paeonia suffruticosa叶部主要病害的病原菌种类及其症状特点,于2016年从洛阳市的4个牡丹种植区采集叶部病害样本,采用组织分离法进行病原菌的分离和纯化,柯赫氏法则回接验证病原菌的致病性,利用形态学与基因序列分析相结合的方法鉴定病原菌的种类。结果表明,洛阳地区牡丹叶部主要病害种类为牡丹红斑病、炭疽病和黑斑病,引起这三种病害的病原菌分别鉴定为枝孢霉菌Cladosporium sp.、胶孢炭疽菌Colletotrichum gloeosporioides和链格孢菌Alternaria alternata。本研究为牡丹叶部病害的快速诊断与防治提供了理论依据。 |
| 英文摘要: |
| In order to identify the symptoms and pathogens of the main leaf diseases of tree peony, infected leaves were collected from four tree peony-growing regions in Luoyang in 2016. The pathogenic isolates were obtained and purified by tissue isolation method. The pathogenicity was proved by the Koch’s rule. The pathogenic isolates were identified based on morphological characteristics and gene sequencing analysis. The results showed that the main leaf diseases on tree peony were red spot, anthracnose and leaf spot. The pathogens of three leaf diseases were identified as Cladosporium sp., Colletotrichum gloeosporioides and Alternaria alternata. This research provides theoretical basis for rapid diagnosis and control of tree peony diseases. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
