| 肖云丽, 张 帆, 徐艳霞, 钟玉林*.两种板栗重要蛀果象甲鉴别研究[J].植物保护,2017,43(4):104-109. |
| 两种板栗重要蛀果象甲鉴别研究 |
| Discrimination of two chestnut fruit borer weevils (Coleoptera: Curculionidae) |
| 投稿时间：2016-09-20 修订日期：2016-11-29 |
| DOI： |
| 中文关键词: 板栗雪片象 栗实象 形态特征 为害特性 生活习性 DNA条形码序列 COⅠ基因 |
| 英文关键词:Niphades castanea Curculio davidi morphological character damaging character living habit DNA barcode sequence COⅠgene |
| 基金项目:湖北省教育厅科学研究项目(D20152904); 湖北省自然科学基金项目(2015CFA158); 湖北省重点实验室项目(2013000703); 湖北省协同创新中心创新团队项目(2015TD06) |
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| 中文摘要: |
| 栗雪片象Niphades castanea Chao和栗实象Curculio davidi Fairmaire幼虫形态相似, 均蛀食板栗果实, 是严重影响板栗产量或质量的重要害虫。为快速准确地鉴别这两种害虫, 采用野外调查法和室内饲养法, 对两种蛀果象甲的形态学和生态学特性进行了观察描述; 同时对基于线粒体COⅠ基因片段的DNA条形码序列进行了提取扩增与分析。结果表明:栗雪片象和栗实象在成虫形态、为害特性和生活习性上差异明显; DNA条形码序列(COⅠ基因5′端684 bp)可作为两种板栗蛀果象甲鉴定的分子标记, 两者具有明显的碱基差异, 遗传距离达18.9%~19.6%, 在系统发育关系上, 栗雪片象与Molytinae亚科的Niphadomimus sp. 聚为一支, 栗实象与Curculio属3个种聚为一支。研究结果可为两种板栗蛀果象甲幼虫的鉴别提供可靠依据, 为象甲科昆虫的进一步研究提供形态、生态和分子证据。 |
| 英文摘要: |
| The larvae of two weevils, Niphades castanea Chao and Curculio davidi Fairmaire, are similar in morphology, and both bore and feed chestnut fruits, seriously affecting the yield and quality of chestnut production. To identify these two pests quickly and accurately, the morphological and ecological characteristics of the two fruit borer weevils were described based on the field survey and indoor breeding method; at the same time, the DNA barcode, i.e., mitochondrial COⅠ gene fragment sequence, was analyzed. Obvious differences existed in adult morphology, damage characters and living habits between N.castanea and C. davidi; the DNA barcode sequence of COⅠ gene fragment, as a molecular marker, could discriminate the two chestnut fruit borer weevils, with obvious difference among the 684 bp COⅠ gene fragment, and the genetic distance reached 18.9%-19.6%. In the phylogenetic tree, N.castanea was clustered with Niphadomimus sp., and C. davidi was clustered with C. elephas, C. venosus and C. sikkimensis. These data provide a tool for distinguishing the two chestnut fruit borer weevils, and the morphological, ecological, and molecular evidences help for the further study of curculionidae insects. |
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